dextran hydrodynamic radius{ keyword }

Int J Food Sci Nutr. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Critical parameters for gel permeation In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Biophysical Journal, 87, 4259-4270. Gillespie, T.; Hulme, M. A. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. The genus Weissella: taxonomy, ecology and biotechnological potential. Epub 2012 May 28. 1905/05) for their financial support. Drug Discov Today Technol. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. A solvent at this temperature is called a theta solvent [53]. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. De Belder, A. N. (2001). Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. The Mark-Houwink a constant is close to 0.5 in poor solvents. Macromolecules, 33, 5730-5739. Learn More. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Intrinsic viscosity molecular weight relationship. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Chem. 2023 BioMed Central Ltd unless otherwise stated. Springer Editions. J Cosmet Laser Ther. Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Durand, A. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Transdermal drug delivery: overcoming the skins barrier function. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Guner, A.; Kibarer, G. (2001). The term f/f0 is sometimes denoted as P, Perrin constant. Viswanath, D.S. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Increased epidermal laser fluence through simultaneous ultrasonic microporation. In addition, we evaluated the regeneration of SC after microporation. Polysaccharides-based nanoparticles as drug delivery systems. Dilute solution properties and degree of chain branching for dextran. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. Ibrahim O, Munavalli GS, Dover JS. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. 7 8 The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). Food Funct. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Carbohydrate Polymers, 39, 377-380. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with Trop J Pharm Res. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Pavlov, G.M. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Article 2012;29(7):201729. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. J Pharm Sci. ; Ross-Murphy, S.B. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. Molecular weight, molecular weight distribution and molecular size of a native dextran. Before The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Hennink, W.E. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Journal of Polymer Science, 17, 527-546. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. 2012;101(8):28619. However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of 4h after microporation, the sizes of the micropore decreased slightly. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Carbohydrate Polymers 88 1440-1444. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. (2013). 2008;26(11):12618. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Masuelli, Martin Alberto (2013). where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Curvale, R.; Masuelli, M.; Perez Padilla, A. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. 2003;10:33847. Dynamics of a Polymer Solution in a Rigid Matriz. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Dextran. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Study of temperature influence. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. Charge: Anionic. Huggins, Maurice L. (1942). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Webwhittier union high school district superintendent. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. ; Hogan, M.P. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Search Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. (2008). On single point determination of intrinsic viscosity. Stenekes, R.J.H. Journal of American Chemical Society, 64(11), 2716-2718. Webof overall macromolecular geometry and hydrodynamic draining. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). J. Careers. 1984;17(6):11703. Carbohydrate Polymers 87, 405-410. The mechanism of the dextran-induced red blood cell Aggregation. Carbohydrate Research, 340, 811-826. Macromolecules, 31, 7712-7716. Probiotics Antimicrob Proteins. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. TEWL measurements were taken 30min after microporation of the human and pig skin. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Gekko, K. (1971). 151-166. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. WebDextran 40 Pharmaceutical Quality. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows These studies were carried out at the Hisko (Gunpo, Korea). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. ; Rjumtsev, E.I. Biomaterials Research Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Determination of polymer structure by Gel Permeation Chromatography. Environ Toxicol Pharmacol. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. ; Weisz, J. Advanced Drug Delivery Reviews, 60, 1650-1662. (1999). Optical properties of dextran in solution and in films. All authors read and approved the final manuscript. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. 4K and M.W. ; De Jong, S.J. PMC WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Determination of intrinsic viscosities of macromolecules and nanoparticles. The authors declare that they have no competing interests. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. @ Nilimesh See attached. WebR H Hydrodynamic radius [m] The basic DLS setup. Carbohydrate Polymers, 68, 477-488. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Pharmacol Pharm. ; Wood, P.J. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). 2012;87(6):1728. This situation is mainly the result of using dextans, solvent, additives, etc. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. 2012;27(3):20212. The https:// ensures that you are connecting to the Journal Argentine Chemical Society 100 (2013) 25-34. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Saudi Pharm J. Intrinsic viscosity of bovine serum albumin conformers. Pharm Res. Comparison of single-point and dilution procedures. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Polymer molecular weight distribution. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. National Library of Medicine Pharm Res. For Research Use Only. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. (2007). Masuelli, Martin Alberto. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Progress in Biophysical Molecules Biological, 68, 207-262. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Fixable: Fixable via Lysine. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. 8. 2004;25(9):155967. Citation-(RIS 2011;5(2):95132. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the 2016;47:15964. 2010;4(1):117. Ioan, C.E. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. 2019;26(1):25261. 2006;3(3):37385. Google Scholar. Garcia de la Torre, J.; Carrasco, B. Biomater Res 24, 22 (2020). Hindered diffusion of dextran and ficoll in microporous membranes. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Recent Pat. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. FOIA MeSH terms Journal of Physical Chemistry. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Bahary, W.S. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. (2003). (2008). Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. International Journal of Pharmaceutics, 277, 99-104. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Smith, David R.; Raymonda, John W. (1972). Walkenhorst, S.; Olivier, R. (2003). GYA primarily conducted research. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. AATEX. Dextrans can function efficiently as anterograde or retrograde tracers, Sung-Wook Choi. Analytical Chemistry. Drug Deliv. Drug deliv. Biophysical Journal, 27, 177-181. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. The Viscosity of Dilute Solutions of Long-Chain Molecules. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. They concluded that a power law behavior seems to be approached only in the small molecular weight region. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. The human skins were from the back or thigh of a 24-year-old woman. (2003). In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. CAS Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution.

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